Generation of induced pluripotent stem cells using recombinant proteins.
نویسندگان
چکیده
Plasmids construction Codons of human Pou5f1/Oct4 (NP_002692), Sox2 (NP_003097), Klf4 (NP_004226), and cMyc (NP_002458) were first optimized for high level protein expression in E. coli. They were then synthesized using DNA oligo based, PCR gene assembling method. Poly-arginine tag plus a linker sequence, i.e. ESGGGGSPGRRRRRRRRRRR, was added to each protein C-terminal. The final DNA fragment was flanked with Nde I and Xho I sites, and inserted into pET41a expression vector Nde I–Xho I sites for protein expression. Each plasmid was verified by DNA sequencing.
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عنوان ژورنال:
- Cell stem cell
دوره 4 5 شماره
صفحات -
تاریخ انتشار 2009